| | Firm | Product(s)/Service(s) | Emp. | Year Established | |
| | Owensboro | |
| Audubon Area Community Services | Social Services Agency | 488 | NA | | | Barton Brands Ltd | Whiskey, gin, vodka, wines, brandy & cocktail bottling | 204 | 1869 | | | BellSouth Telecommunications | National directory call center (1+411) | 200 | 1997 | | | Dana Corporation | Steel pickup truck frames (Tundra and Sequoia) | 380 | 1997 | | | Daramic Inc | Automotive deepcycle & Industrial battery separators | 264 | 1959 | | | HON Co | Wooden office chairs | 260 | 1945 | | | Kimberly-Clark Corp | Tissue paper & towels | 342 | NA | | | Miles Farm Supply LLC | Agricultural distribution center | 175 | 1973 | | | MPD Inc | Commercial tubes & components, microwave & police radar equipment, breath analyzers, ceramics & ceramic to metal seals | 264 | 1987 | | | Omico Plastics Inc | Blow molded plastic parts | 185 | 1964 | | | Plastech | Plastic blow molded automotive parts | 200 | 1974 | | | Southern Star Central Gas Pipeline Inc | Headquarters | 456 | 2002 | | | Specialty Foods Field Packing Division | Processed meat products | 600 | 1915 | | | Swedish Match North America | Smokeless tobacco products | 356 | 1973 | | | Texas Gas Co | Headquarters, pipeline co. | 400 | 1960 | | | Toyotetsu Mid America LLC | Stamped and welded metal automotive parts | 561 | 2002 | | | Unifirst | Uniform distribution | 327 | 1998 | | | Unilever Foods North America | Pasta and simmer sauces | 393 | 1974 | | | US Bank Home Mortgage | Loan processing and underwriting service center, loan servicing center | 685 | 1992 | | | Yager Materials LLC | Ready-mixed concrete, crushed limestone, sand & gravel | 192 | 1917 | |